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. Author manuscript; available in PMC: 2009 Sep 24.
Published in final edited form as: Anal Chem. 2009 Jul 1;81(13):5373–5380. doi: 10.1021/ac900889p

Fig. 6. Probe: Anti-Biotin.

Fig. 6

Simultaneously measured binding curves vs. probe concentration for reaction of monovalent Fab fragments of anti-biotin mouse IgG with (a) 40× loaded biotin-BSA; (b) 20× loaded biotin-BSA; (c) 10× loaded biotin-BSA; (d) 5× loaded biotin-BSA; (e) 4% loaded biotin-PVA; (f) 2% loaded biotin-PVA. For probe concentration, (1) open squares: 27 nM; (2) solid circles: 80 nM; (3) open up-pointing triangles: 240 nM; (4) solid down-pointing triangles: 480 nM. The curves in each panel were fitted globally using a two-site Langmuir reaction model (shown in dashed lines). The fitting parameters are listed in Table 2.