Figure 5.

Spike-dependent regulation of GABA release onto POMC neurons by hypocretin. A, B, Traces from a representative experiment (A) and summary of nine cells (B) showing IPSC frequency before and during hypocretin application. C, IPSC waveforms detected in the same experiment presented in A. Individual events are shown in gray, and the mean is shown in black. Note the increased IPSC amplitude in the presence of hypocretin. D, Averaged effect of hypocretin on IPSC amplitude (n = 9). E, F, Lack of hypocretin effect is shown either for mIPSC frequency (E) or amplitude (F) in six POMC neurons. Hcrt, Hypocretin; Ctrl, control. *p < 0.05.