Secondary structure of anti-SA as monitored by far UV-CD in the presence or absence of 1.5% silicone oil under the following solution conditions: (●), A5 buffer, pH 5.0; (■), A5NaCL, pH 5.0; (▲), phosphate, pH 7.4; (◆), PBS pH 7.4. Open symbols were without silicone oil; closed symbols were with silicone oil. For each solution condition, the spectrum shown is an average of two spectra acquired for the protein.