Figure 2.

GFP expression in the liver of adult nestin-GFP transgenic mice after CCl₄ treatment. Cell nuclei are stained with DAPI (blue).

A – Aggregates of GFP-positive cells in the periportal area of the liver 3 days after a single administration of CCl₄.

B – Growing primitive ductular structures after 4 rounds of CCl₄ treatment contain large number of GFP-expressing cells.

C – A poorly organized ductular structure containing GFP-expressing cells after 4 rounds of CCl₄

D – A small aggregate of GFP-expressing cells without an obvious ductular organization seen after 4 rounds of CCl₄ treatment.

Scale bar is 20 μm in A, C and D; 40 μm in B.