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. 2009 Sep 6;9:315. doi: 10.1186/1471-2407-9-315

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Copyright ©2009 Wu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cripto-1 knock-down inhibited the invasion of CNE-2 cell lines. Effects of Cripto-1 knockdown on cell invasive potency were determined by an in vitro invasion assay. (A) 48 hr after seeded, the cells on the upper chambers were removed, and the invasive cells on the lower surface of the membrane were fixed and stained with hematoxylin, and visualized at ×200 magnification. a: CNE-2, b: CNE-2/GFP⁺/mock, c: CNE-2/GFP⁺/Cripto-1^-. (B) The cells on the lower surface of the membrane were counted in five randomly selected fields. Each value represents the average of triplicate determinations expressed as the mean ± SD. (* p < 0.01).