Table 4.
PCR and qRT-PCR primers used in this study.
| Primer | Sequence (5'→3') | Description |
|---|---|---|
| C42/F | CCCAAAGCTTGGGTCCTGCTCATCACCAGAAACTCCA | HindIII site, used to construct pKH11 |
| CF2/R | GCTCTAGAGCAACCGCTTTCAGGCACTGTTGTTC | XbaI site, used to construct pKH11 |
| Primers for RT-PCR--asterisk denotes primer for cDNA synthesis | ||
| Primer | Sequence (5'→3') | Gene |
| MQO RT/A* | AGGCCTGCCCGTAGACTTTC | Arth_4255 |
| MQO RT/B | TCTTCACCGCCGGTATGAG | Arth_4255 |
| ChrB RT/A* | CGAGGATGAGGGATCGTTTG | Arth_4254 |
| ChrB RT/B | TGATCCGCAGGAACATCG | Arth_4254 |
| SP RT/F | CCCGGGAGCACTTCGACTGGA | Arth_4253 |
| SP RT/R* | CCTGGCGCGTTCGGTTGCAT | Arth_4253 |
| Cog4RT/F | AAGGCCTACGTCTCCAACGAACA | Arth_4252 |
| Cog4RT/R* | ATTCTGTCGGTGACCGTGTCAGT | Arth_4252 |
| ChrAP RT/A | GCTTCATCCTGTGCTTCTTG | Arth_4251 |
| ChrAP RT/B* | TGTTCATGATGCCGGTACTG | Arth_4251 |
| BP RT/F | CCTGCGCCGCTACGAACTCACCGAT | Arth_4249 |
| BP RT/R* | GCGCTGGTGTTCGTACAGCCCGTC' | Arth_4249 |
| ChrAC RT/A* | AAGTACAGGGCCAGGTTC | Arth_4248 |
| ChrAC RT/B | TGCGGTCCTGTCCTATATC' | Arth_4248 |
| Lppy RT/F | AGTGACCACGGCCATCAATTTCCA | Arth_4247 |
| Lppy RT/R* | TCAGGGAATGATTGTGCACGGAGA | Arth_4247 |