Table 2.
Differential gene expression by microarray and RT-PCR analysis
Microarray analysis | RT-PCR | ||||
Sequence | Fold H/C | Fold (H/C) | Fold (L/C) | Fold (H/L) | |
Genes up-regulated by ACTH treatment | |||||
ARL10 | 7 | H = L > C | 9 | 4 | NS |
ASAL | 5.6 | H = L > C | 31 | 7 | NS |
PRAX-1 | 3.8 | H = L > C | 5 | 5 | NS |
C-TSK | 3.4 | H > L > C | 57 | 14 | 4 |
ICER | 3.4 | H > C, C = L, L = H | 12 | NS | NS |
HSD17B7 | 3.2 | H > C, H > L, C = L | 14 | NS | 4 |
STAR | 2.3 | H = L = C | NS | NS | NS |
SC4MOL | 1.5 | H = L = C | NS | NS | NS |
PISD | 2.6 | H = L > C | 3 | 3 | NS |
ATF4 | 1.5 | H = L = C | NS | NS | NS |
FKBP | 1.4 | H = L = C | NS | NS | NS |
Genes down-regulated by ACTH treatment | |||||
MYPN | 3.2 | H < L, H < C, L = C | 15 | 7 | NS |
KCTD1 | 2.3 | H < L, H < C, L = C | 3 | NS | 3 |
NARFL | 2 | H = L = C | NS | NS | NS |
NCB5R | 1.4 | H = L, H < C, L = C | 2 | NS | NS |
Genes differentially expressed in high and low ACTH response groups | |||||
NUDT5 | 0.65 | H = L = C | NS | NS | NS |
AGT2 | 0.53 | H = C = L, L > C | NS | 2 | NS |
CCNG1 | 1.23 | H = L = C | NS | NS | NS |
PRAX-1 | 1.32 | H = L, H > C, L > C | 5 | 5 | NS |
NS: No statistical significant difference is shown between compared groups
H: High responder group
L: Low responder group
C: Control group