Figure 4.

Real-time qPCR validation of differential expression in vitellogenic vs. previtellogenic ovaries (A), mature vs. vitellogenic ovaries (B), and atretic vs. vitellogenic/mature ovaries (C). Bar graphs represent relative fold change (FC) of selected transcripts obtained with the microarray (black bars) or by qPCR (with bars). The mean FC ± SEM are shown for each gene. The qPCR and microarray experiments were both conducted on RNA extracted from the same three female individuals for each developmental stage examined. See Tables 1, 2 and 3 for transcript abbreviations.