Table 1. Primary antibodies used for immunohistochemical analysis of the pig retina.
Antibody | Cell specificity | Host | Dilution | Source | Reference |
---|---|---|---|---|---|
Nestin |
neural stem/progenitor cells, Müller glia |
mouse |
1:400 |
BD Biosciences |
[25,41,44] |
Pax6 |
retinal progenitors, ganglion, amacrine, and horizontal cells |
rabbit |
1:1,000 |
Chemicon |
[28,45] |
Ki67 |
proliferating cells |
mouse |
1:300 |
BD Biosciences |
[46] |
Brn3a |
ganglion cells |
mouse |
1:40 |
Chemicon |
[47] |
Neurofilament (NF)-160 |
ganglion, horizontal, and bipolar cells |
mouse |
1:350 |
Sigma |
[22,38,48,49] |
AP2α |
amacrine cells |
mouse |
1:50 |
Developmental Studies Hybridoma Bank |
[26] |
Calretinin |
ganglion, amacrine, horizontal, cone photoreceptor, and bipolar cells |
mouse |
1:500 |
Chemicon |
[33,50] |
Calbindin |
horizontal, ganglion, amacrine, bipolar, and cone photoreceptor cells |
rabbit |
1:1,500 |
Chemicon |
[27,51,52] |
GAD65 |
GABAergic amacrine, rare horizontal ganglion cells |
mouse |
1:1,000 |
Developmental Studies Hybridoma Bank |
[27,28] |
Islet1 |
amacrine, bipolar, ganglion, and horizontal cells |
mouse |
1:500 |
Developmental Studies Hybridoma Bank |
[29,37] |
PKCα |
rod bipolar, amacrine, and ganglion cells, cones |
mouse |
1:400 |
Sigma |
[27,30,53] |
Recoverin |
photoreceptors, cone bipolar cells, rare ganglion cells |
rabbit |
1:1,000 |
K. Koch |
[42,51,54] |
Rhodopsin (Rho4D2) |
rods |
mouse |
1:100 |
R. Molday |
[2,36,43] |
GNAT2 |
cones |
rabbit |
1:500 |
Santa Cruz Biotechnology |
[55-57] |
P75 neurotrophin receptor |
Müller glia and ganglion cells |
rabbit |
1:350 |
Promega |
[31,32,58] |
Glutamine synthetase |
Müller glia |
mouse |
1:500 |
BD Biosciences |
[35,59] |
GFAP | astrocytes | rabbit | 1:500 | DAKO | [60-62] |
The cell specificity is described according to the published literature (listed in the reference column) on the antibodies to the same proteins. Details for the primary antibodies (host, dilution used and the source) used in our study are presented.