Skip to main content
. 2009 Sep 21;15:1915–1928.

Table 1. Primary antibodies used for immunohistochemical analysis of the pig retina.

Antibody Cell specificity Host Dilution Source Reference
Nestin
neural stem/progenitor cells, Müller glia
mouse
1:400
BD Biosciences
[25,41,44]
Pax6
retinal progenitors, ganglion, amacrine, and horizontal cells
rabbit
1:1,000
Chemicon
[28,45]
Ki67
proliferating cells
mouse
1:300
BD Biosciences
[46]
Brn3a
ganglion cells
mouse
1:40
Chemicon
[47]
Neurofilament (NF)-160
ganglion, horizontal, and bipolar cells
mouse
1:350
Sigma
[22,38,48,49]
AP2α
amacrine cells
mouse
1:50
Developmental Studies Hybridoma Bank
[26]
Calretinin
ganglion, amacrine, horizontal, cone photoreceptor, and bipolar cells
mouse
1:500
Chemicon
[33,50]
Calbindin
horizontal, ganglion, amacrine, bipolar, and cone photoreceptor cells
rabbit
1:1,500
Chemicon
[27,51,52]
GAD65
GABAergic amacrine, rare horizontal ganglion cells
mouse
1:1,000
Developmental Studies Hybridoma Bank
[27,28]
Islet1
amacrine, bipolar, ganglion, and horizontal cells
mouse
1:500
Developmental Studies Hybridoma Bank
[29,37]
PKCα
rod bipolar, amacrine, and ganglion cells, cones
mouse
1:400
Sigma
[27,30,53]
Recoverin
photoreceptors, cone bipolar cells, rare ganglion cells
rabbit
1:1,000
K. Koch
[42,51,54]
Rhodopsin (Rho4D2)
rods
mouse
1:100
R. Molday
[2,36,43]
GNAT2
cones
rabbit
1:500
Santa Cruz Biotechnology
[55-57]
P75 neurotrophin receptor
Müller glia and ganglion cells
rabbit
1:350
Promega
[31,32,58]
Glutamine synthetase
Müller glia
mouse
1:500
BD Biosciences
[35,59]
GFAP astrocytes rabbit 1:500 DAKO [60-62]

The cell specificity is described according to the published literature (listed in the reference column) on the antibodies to the same proteins. Details for the primary antibodies (host, dilution used and the source) used in our study are presented.