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. Author manuscript; available in PMC: 2009 Sep 24.
Published in final edited form as: Anal Chem. 2008 Mar 22;80(9):3198–3204. doi: 10.1021/ac800362e

Figure 3.

Figure 3

Fluorescence signal intensity of products as a function of enzyme-substrate reaction time for different trypsin concentrations ranging from ctrypsin = 1 ng/mL and ctrypsin = 1 μg/mL without preconcentration. BODIPY FL casein with csubstrate = 50 μg/mL was used as a fluorogenic substrate to monitor the turnover rate. It shows that the limit of detection was between ctrypsin = 1 ng/mL and ctrypsin = 10 ng/mL (> 10% change of the signal intensity above control) and the reaction time required to turn over enough substrates was ~ 1 hours.