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. Author manuscript; available in PMC: 2009 Sep 24.
Published in final edited form as: Anal Chem. 2008 Mar 22;80(9):3198–3204. doi: 10.1021/ac800362e

Figure 5.

Figure 5

Reaction rate versus preconcentration time, calculated from the fitting curve (Figure 4). In case of ctrypsin = 1 ng/mL, the initial slope was drastically increased through preconcentration (compare with the grey line (open circle) which shows the slope curve of the same concentration without preconcentration). At ctrypsin = 100 pg/mL, the initial slope was still higher than any of those measured without preconcentration. In case of the lowest concentration tested, ctrypsin = 10 pg/mL, the initial reaction rate was comparable to the initial slope of ctrypsin = 10 ng/mL without preconcentration.