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. 2009 Oct 6;4(10):e7215. doi: 10.1371/journal.pone.0007215

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Verkoczy et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Representative histograms showing staining of 10⁶ splenocytes from unimmunized C57BL/6 and BALB/c inbred strains (congenic for IgH^a and IgH^b allotypic determinants) with 100 ng of gp41 MPER or clade B V3-specific APC-labeled tetramers. Numbers indicate the percentage of tetramer-reactive cells within singlet, live, CD19⁺-gates. Results are representative of several experiments; similar results were seen in B cells from BM, LN, and PBL (not shown).