Table 1. Base substitution specificity of allelic variant W438 of human Polλ during short gap filling synthesis.
| Enzyme | Mispair | # mutantsc | E.R.d×10−4 |
| Polλ R438 a | T • dGTP | 6 | 2.5 |
| T • dCTP | 0 | ≤0.4 | |
| T • dTTP | 0 | ≤0.4 | |
| G • dATP | 0 | ≤0.4 | |
| G • dGTP | 0 | ≤0.4 | |
| A • dCTP | 31 | 13 | |
| A • dGTP | 1 | 0.4 | |
| A • dATP | 1 | 0.4 | |
| Polλ W438 b | T • dGTP | 26 | 10 |
| T • dCTP | 0 | ≤0.4 | |
| T • dTTP | 0 | ≤0.4 | |
| G • dATP | 9 | 3.5 | |
| G • dGTP | 0 | ≤0.4 | |
| A • dCTP | 57 | 22 | |
| A • dGTP | 3 | 1.2 | |
| A • dATP | 0 | ≤0.4 |
a
Mutation Frequency (Polλ R438): 9.8×10−4.
b
Mutation Frequency (Polλ W438): 22.05×10−4.
c
Total sequenced: Polλ R438->42; Polλ W438->96.
d
The error rates are calculated based on sequencing mutants from two different reactions with each enzyme, and using the average mutant frequency for those two experiments, i.e., 9.8×10−4 and 22.05×10−4 for Polλ R438 and Polλ W438, respectively.