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. 2009 Aug;21(8):2357–2377. doi: 10.1105/tpc.108.062992

Figure 5.

Figure 5.

PA Activates NADPH Oxidase Activity in GCPs and the Plasma Membrane.

(A) The effect of PA on NADPH oxidase activity in GCPs. GCPs were incubated with XTT dye together with 1 μM 16:0-18:2 PA,16:0-18:2 PC, or LysoPA or 10 μM ABA at 25°C for 10 min. After centrifugation, the supernatant was used for spectrophotometric analysis of XTT formazan production at A470. The NADPH activity calculation is described in Methods.

(B) The effect of PA on NADPH oxidase activity in the plasma membrane. Plasma membrane vesicles (3 to 6 μg protein) were incubated with XTT, together with 1 μM 16:0-18:2 PA,16:0-18:2 PC, or 10 μM ABA at 25°C for 10 min. The reaction solution was used for spectrophotometric analysis of XTT formazan production at A470. NADPH oxidase activity was expressed as ΔA 470 per mg protein per min (ΔA 470 represents the difference of XTT formazan absorbance at 470 nm in the presence and absence of superoxide dismutase [SOD]).

The data are means ± se of three independent experiments. The asterisk in (A) and (B) indicates that the mean value is significantly different from that of the control (P < 0.05).