Figure 3.

PFT1 Is a Positive Regulator of JA-Dependent Defenses in Arabidopsis.

(A) to (H) Expression from JA-responsive genes PDF1.2, HEL, CHIB, ESP, VSP, OPR, MYC2, and WRKY70 was examined by quantitative RT-PCR in wild-type (Col-0) and pft1 mutants 6 h following MeJA treatment.

(I) The root lengths of pft1, OX1, and G1 seedlings germinated on half-strength Murashige and Skoog (MS) media containing either 0 or 5 μM of MeJA were measured 6 d after germination. Error bars represent the se from three independent experiments that included ∼10 seedlings each.

(J) Gene expression of PAL1 was examined in 3- to 4-week-old, MeJA-treated wild-type (Col-0), pft1, G1 (genomic complement) , and OX1 (35S:PFT1) plants. The expression data were normalized relative to the expression level of β-ACTIN (See Methods) and presented logarithmically for PDF1.2. Error bars in (A) to (H) and (J) represent se from three independent experiments that contained at least 20 plants each.

(K) To visualize anthocyanin accumulation, 2-week-old soil-grown plants of wild-type, pft1, and OX1 were incubated in water until visible anthocyanin developed in the leaves.

[See online article for color version of this figure.]