Figure 5.

PFT1 Function Is Conserved in Plants.

(A) Transgenic expression of Ta PFT1, a PFT1 homolog from wheat, in the pft1 mutant background complements the compromised defense gene expression.

(B) The 35S:TaPFT1/pft1 line shows spontaneous lesion development (arrow).

(C) and (D) Expression of Ta PFT1 complements the increased F. oxysporum resistance of the pft1 mutant.

(E) and (F) Expression of Ta PFT1 complements the delayed flowering phenotype of pft1. PDF1.2 transcript levels were examined by quantitative RT-PCR, and the expression data were normalized relative to β-ACTIN expression and presented logarithmically.

Numbers on each bar in (A) represent fold difference in expression of PDF1.2 in untreated plants of pft1 and 35S:TaPFT1/pft1 relative to untreated wild-type plants. Error bars represent se from three independent replicates that contained ∼20 plants each. F. oxysporum inoculation experiments were conducted as described above, and symptom development was scored 10 d after inoculation.