Figure 7.

The pft1 med8 Double Mutant Shows an Additive Increase in Both the Flowering Time and the Pathogen Defense Phenotypes Compared with the pft1 and med8 Single Mutants.

(A) The expression of PDF1.2 is greatly reduced in the double mutant relative to the wild type and pft1 and med8 single mutants in both untreated and MeJA-treated plants.

(B) and (C) The double mutant also shows reduced symptoms and greater survival after inoculation with F. oxysporum.

(D) The flowering time of the double mutant is more delayed than the individual single mutants. PDF1.2 transcript levels were examined by quantitative RT-PCR, and the expression data were normalized relative to β-ACTIN expression and presented logarithmically. The disease symptoms and survival were assessed 14 and 24 d after inoculation, respectively. The error bars from the F. oxysporum inoculation and defense gene experiments are the se from three replicates of ∼30 plants each, whereas the error bars for the flowering time experiment are the se from 18 plants and are representative of two independent replicated experiments.

[See online article for color version of this figure.]