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. 2009 Aug;21(8):2458–2472. doi: 10.1105/tpc.107.056044

Figure 1.

Figure 1.

AvrPto Caused the Proteolysis of Sl RIN4 in Tomato.

(A) AvrPto- and AvrPtoB-triggered degradation of RIN4 was dependent on Pto and Prf in tomato. 3HA:SlRIN4 and AvrPto, AvrPtoB, or empty vector (EV) were transiently expressed using A. tumefaciens in RG-76R tomato plants and RG-76R plants with mutations in Pto (RG-pto11) or Prf (RG-prf3). Twenty-four hours after infiltration, samples were collected and immunoblotted using anti-HA (α-HA) antibody to detect the presence of Sl RIN4. LC, silver staining of the membrane to show equal loading.

(B) AvrPto caused the proteolysis of Sl RIN4 expressed from its native promoter in tomato. AvrPto (+) or empty vector (−) were transiently expressed by A. tumefaciens in RG-76R tomato plants. Twenty-four hours after infiltration, samples were collected and immunoblotted using anti-At RIN4 (α-RIN4) antibody to detect native Sl RIN4. LC, Ponceau S staining of the membrane to show equal loading.