Figure 4.

Infection Phenotype of the ssd1 Mutant in SIPK WIPK–Silenced Plants.

(A) Conidial suspension (1 × 10⁶ conidia/mL) of the ssd1 mutant RCD1 was sprayed onto leaves of control (empty vector) or SIPK- and WIPK-silenced plants. Typical anthracnose lesions were produced on leaves of SIPK- and WIPK-silenced plants, while lesions were absent from leaves of control plants at 5 DAI. Bar = 1 cm.

(B) Quantification of papilla and infection hypha formation at sites of attempted penetration by C. orbiculare appressoria. At 72 HAI, leaves inoculated with the wild-type 104-T and ssd1 mutant RCD1 were stained with aniline blue to detect callose and infection hyphae. At least 200 appressoria were counted for each fungal strain, and standard errors were calculated from three replicate experiments.

[See online article for color version of this figure.]