Figure 6.
Kinase Activity of SIPK and WIPK during Infection.
Conidial suspension (1 × 106 conidia/mL) of the wild-type strain 104-T, complemented strain RCDcm1, or ssd1 mutants RCD1 and RCD2 was sprayed onto N. benthamiana leaves. At time points of 0 h (no inoculation) and 3, 6, 12, 24, and 48 HAI, proteins were extracted from the leaves of inoculated plants and subjected to an immunocomplex kinase assay. The graph at the right side of each panel indicates the relative kinase activity calculated by comparison with activity at 0 h. Loading controls represent Coomassie blue staining of the ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (RBCL). Kinase activity of 104-T and RCD1 was determined in three replicated experiments. One set of representative data is shown in this figure. Kinase activity of RCDcm1 and RCD2 was determined in a single experiment.