Figure 3.

The Δssb1Δssb2 strain displays glucose-specific growth defects and does not normally enter stationary phase. (A) Growth curves of wild type (circles, solid line) and the Δssb1Δssb2 strain (squares, dotted line) on glucose-containing medium prior and after the diauxic shift in batch culture. Doubling times (see the Results) were calculated independently for the early exponential (between 4 and 10 h) and the early stationary phase (between 26.5 and 55 h). (B) Samples taken after 24 h of growth (OD₆₀₀ 7.2–7.3) were analyzed microscopically. The ratio of budded cells versus unbudded cells was determined using a Neubauer improved counting chamber. (C) Growth curves of wild type (circles, solid line) and the Δssb1Δssb2 strain (squares, dotted line) logarithmically growing on ethanol-containing medium.