Figure 7.

XHYLS-1 is required for apical targeting/anchoring of basal bodies. (A) Representative images of control embryos and embryos depleted of XHYLS-1, Polaris or IFT-52, stained with antibodies to acetylated tubulin and γ-tubulin to illustrate effect on basal body distribution. Images are x–y projections of deconvolved 3D data stacks. (B) x–z projection of 3D data stacks presented in A and Figure 6C. (C) Model for the role of HYLS-1 in cilia formation. HYLS-1 is recruited to the forming centriole by its association with SAS-4, and like SAS-4 is locked into the centriole during the assembly of centriolar microtubules. Incorporation of HYLS-1 confers on centrioles the capacity to initiate ciliogenesis. Bars, 10 μm.