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. 2009 Sep 15;23(18):2237–2251. doi: 10.1101/gad.536109

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Figure 4. — Ipl1 promotes SC disassembly in cdc5-mn and CDC5-IN cells. Examples of nuclei and quantification of Zip1 staining from cdc5-mn (Y1657, A,C) and cdc5-mn ipl1-mn (Y2030, B,D) mutants. (E,F) Analysis of Zip1 SC disassembly in a time course using a ndt80Δ strain with inducible CDC5 (CDC5-IN, Y2262, E) expression by addition to β-estradiol (+ED, at 7 h) and in an ndt80Δ CDC5-IN ip1-mn strain (Y2263, F). At least 100 nuclei were scored for each stage. Zip1 protein levels are shown for ndt80Δ CDC5-IN (G) and ndt80Δ CDC5-IN ip1-mn (H) strains.