Skip to main content
. 2009 Sep 15;23(18):2140–2151. doi: 10.1101/gad.1820209

Figure 6.

Figure 6.

Constitutive miR-200 expression converts 2D morphology, blocks TGFβ reponsiveness, and abrogates in vitro migration and invasion. (A) 344SQ cells grown on tissue culture plastic. (B) 344SQ cells treated for 10 d with TGFβ. (C) 344SQ_200b_1 transfectants on tissue culture plastic. (D) 344SQ_200b_1 transfectants treated for 10 d with TGFβ. (E) 344SQ_vector cells grown on Matrigel. (F) 344SQ_vector cells grown on Matrigel and stimulated with TGFβ for 5 d. (G) 344SQ_200b_1 cells grown on Matrigel. (H) 344SQ_200b_1 cells grown on Matrigel and stimulated with TGFβ for 5 d. (I,J) 344SQ_200b_1 cells grown on Matrigel were imaged by confocal microscopy after staining for ZO-1 (green), α6-integrin (red), and Topro-3 (blue). Bar, 50 μm. (K) In vitro Transwell migration (black) and invasion (gray) assays for the three cell lines. (L–Q) Q-PCR analysis of indicated EMT-related genes at baseline (black) or after TGFβ treatment (gray) for the 344SQ_vector cells or two different 344SQ_200b transfectants grown in monolayer culture as in A–D.