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. 2009 Sep 15;23(18):2201–2209. doi: 10.1101/gad.1825809

Figure 4.

Figure 4.

Intracellular heme concentration modulates PGC-1α gene expression. (A) Succinylacetone induces the expression of PGC-1α and ALAS1. HepG2 cells were treated with succinylacetone (5 mM) for 16 h. mRNA were quantitated by RT–PCR and normalized to GADPH. (B) Heme represses Alas1 and PGC-1α expression in HepG2 cells. Heme treatment was 10 μM for 16 h. (C) Heme represses Alas1 and PGC-1α in primary mouse hepatocytes. Heme treatment was 6 μM for 16 h. (D) Effect of heme on the expression of PGC-1α gene in cells depleted of Rev-erbα in HepG2 cells. Mean ± SD (n = 3); (*) P < 0.05 compared with DMSO-treated cells transfected with control shRNA. (E) Effect of heme on the occupancy of Rev-erbα, HDAC3, and NCoR at the PGC-1α intronic sequence in HepG2 cells. Results of heme treatment are normalized to DMSO results.