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Dual color FISH analysis of HER2 in cholangiocarcinoma specimens. A: Nonamplified tumor with single HER2 gene copy status. The red probe is specific for HER2. The green probe is specific for chromosome 17 centromere; B: Tumor with high amplification of the HER2 gene. There are multiple red HER2 specific signals in this tumor in relation to the green chromosome 17 signals.
