Skip to main content
. 2009 May 18;86(4):769–778. doi: 10.1189/jlb.0708452

Figure 5.

Figure 5.

IL-4 and IL-13 production from basophils is p38α-dependent. Basophils were stimulated for 15 min (A) and 24 h (B) in the indicated cytokines. Western blot analyses of basophil whole cell lysates were carried out using antibodies to total p38α and phosphotyrosine-specific p38 (p-p38α). Quantitation was performed by densitometry, and phospho-p38α was normalized to total p38α. Fold induction is relative to IL-3 stimulation alone. Blots are representative of three or four independent experiments, quantified in Supplemental Table 2A. (C) Basophils from wild-type (WT) C57BL/6 or MyD88−/− mice were stimulated in the indicated cytokine conditions for 15 min, and then whole cell lysates were analyzed by Western blot. Bands were quantified densitometrically, and phospho-p38 was normalized to total p38. Fold-induction is relative to IL-3 stimulation alone. Blots are representative of three independent experiments. (D) IL-4 and IL-13 production from basophils is reduced in the presence of the p38 inhibitor SB239063. Basophils were stimulated in the indicated cytokine conditions with or without SB239063 (500 nM). Results are the mean of three experiments ± sem (*, P<0.05).