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. 2009 Jul 20;86(4):833–845. doi: 10.1189/jlb.0908551

Figure 1.

Figure 1.

MIP-1β induces CCR5-mediated chemotaxis of primary human macrophages. (A) Macrophages were pretreated for 1 h without (•) or with (□) the CCR5 antagonist M657 (1 μM), placed in Transwell inserts, and stimulated with the indicated concentrations of MIP-1β. Results are expressed as C.I. (B) Migration of macrophages from CCR5Δ32 homozygous donors in response to MIP-1β (1 nM), SDF-1α (1 nM), or fMLP (100 nM). WT, Wild-type. (C) Modified checkerboard analysis of macrophage migration to MIP-1β (1 nM) in the upper and/or lower compartments as indicated. (D) Macrophages were pretreated without or with inhibitors for Gi (PTX, 100 ng/ml) or Gs (NF449, 200 nM) for 2 h prior to measuring migration to MIP-1β. Data shown are means ± se of three independent experiments using cells from different donors (*, P<0.05; **, P<0.01; ***, P<0.001).