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. Author manuscript; available in PMC: 2009 Sep 25.
Published in final edited form as: Dev Biol. 2008 Jul 4;321(2):397–406. doi: 10.1016/j.ydbio.2008.06.033

Figure 4.

Figure 4

Heat shock of Tg(hsp70:ca-fgfr1) embryos causes ectopic FGF signaling and perturbs pectoral fin development. (A,B) In situ hybridization depicts pea3 expression at 32 hpf; dorsal views, anterior to the left. (A) After heat shock at 24 hpf, non-transgenic (non-tg) embryos show a wild-type restriction of pea3 expression to specific regions, including the midbrain-hindbrain boundary and the pharyngeal pouches. (B) In contrast, heat shock of transgenic (tg) embryos causes strong and ectopic expression of pea3. (C,D) Dorsal views at 72 hpf of embryos after heat shock at the 8-somite stage. (C) Non-transgenic embryos exhibit pectoral fins of normal length (bar). (D) Transgenic embryos lack at least one pectoral fin, as shown here (n=14/50), or have two small fins (n=31/50).