Fig. 5.

Antitumor effect of EVn-50 on human prostate, cervical, and liver cancer xenografts. Mice bearing established PC-3 (A), HepG2 (D), or Hela (D) human tumor xenografts were treated with vehicle or EVn-50 (i.p. injection). For positive controls, we used chemotherapy drugs ADM (1 mg/kg) for PC-3, Cisplatin (DDP, 4 mg/kg) for Hela, and 5-fluorouracil (5-FU, 20 mg/kg) for HepG2 cells. Treatment groups received drugs every other day until the termination of the experiment. All mice were sacrificed at day 16 after the first treatment. Each point represents the mean of tumors ± SE. Statistical comparisons for tumor size in treated mice relative to control mice indicate: *P <0.01. B. Histological sections of PC-3 tumors from two control and two treated mice were analyzed for apoptosis (TUNEL assay). The apoptotic index of the tumor cells increased 3.3–3.6 fold (p<0.005) in the EVn-50 treated mice. C. Regulation of Bax and Bcl-2 protein expression in PC-3 tumor xenografts. Tumors from two control and two treated mice were harvested at day 16, 12 hours following the last EVn-50 treatment. Total protein was subjected to Western analyses of Bax and Bcl-2 and normalized with actin.