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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Anal Biochem. 2009 Jul 16;394(1):101–109. doi: 10.1016/j.ab.2009.07.019

Figure 7.

Figure 7

Steady-state kinetics of CD38 utilizing the coupled assay. Reaction mixtures contained 5-320 μM NAD+, 0.2 mM NADH, 1 mM DTT, 3.3 mM α-ketoglutarate, 1-2 μM MBP-PncA, ∼3 units of glutamate dehydrogenase from bovine liver, 2 nM CD38 in 20 mM potassium phosphate at pH 7.5. The rates were measured and analyzed as described under Materials and Methods.