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. 2009 Sep 21;119(10):2898–2913. doi: 10.1172/JCI36498

Figure 7. Tregs facilitate alveolar neutrophil clearance in vivo and in vitro.

Figure 7

(A) After administration of i.t. LPS and AT of PBS sham treatment or the indicated lymphocyte subtypes, the percentage of neutrophils in the BAL on day 10 was assessed. †P < 0.05 vs. sham. (B) Percent neutrophils in the alveolar compartment of WT mice after receiving isotype or Treg-depleting Abs. †P < 0.05 versus isotype Ab. (C) Percent BAL neutrophils undergoing apoptosis, as assessed by dual labeling with Annexin V and 7-AAD on day 4 after i.t. LPS in WT or Rag-1–/– mice receiving PBS or AT of Tregs or CD4+CD25– cells. (D) WT and Rag-1–/– mice were exposed to i.t. LPS, and on day 1 or day 4, both BAL and ex vivo BAL mixed cultures were performed. Neutrophils and macrophages were cultured in vitro for 6 hours, then media or the indicated lymphocyte subset was added in a 1:10 lymphocyte/macrophage ratio. (E) Neutrophil apoptosis or macrophage phagocytosis was assessed 24 hours after addition of the indicated cells in vitro. *P < 0.05 vs. day-1 Rag-1–/– and media only.