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. 2009 Sep 21;119(10):3024–3034. doi: 10.1172/JCI38716

Figure 3. Increased joint inflammation and osteoclastogenesis in TNF-Tg/Nfkb2–/– mice.

Figure 3

(A) Age-related changes in clinically assessed joint deformation scores showed that joint deformation occurred earlier in the TNF-Tg/Nfkb2–/– mice (n = 7) than in their TNF-Tg/Nfkb2+/– littermates (n = 8). (B) Representative TRAP-stained sections (original magnification, ×20) from 12-week-old animals showed more severe wrist joint inflammation (green arrows) and more osteoclasts (yellow arrowheads) in a TNF-Tg/Nfkb2–/– mouse than in a TNF-Tg/Nfkb2+/– mouse. Histomorphometric analysis showed that the area of inflammatory tissue (upper panel) and osteoclast numbers (lower panel) were increased in the wrists of TNF-Tg/Nfkb2–/– mice. *P < 0.05. (C) The percentage of cartilage eroded surface/total joint surface was measured in carpal bones of 6-week-old mice (n = 5/group). (D) Serum levels of murine TNF (black bars) and human TNF (red bars) were tested with ELISA at 6 and 12 weeks of age (*P < 0.05 vs. TNF-Tg/Nfkb+/– littermates).