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. 2009 Sep 14;119(10):3000–3010. doi: 10.1172/JCI38746

Figure 2. EGFR mutant lung tumors display higher levels of the EGFR ligands, Areg and Ereg, compared with normal lungs.

Figure 2

(A) Unsupervised clustering of tumors from C/L858R, C/T790M, and C/L+T animals and normal lungs from control littermates fed a dox-containing diet form 2 separate groups. “Normal-1” lungs were derived from animals on a pure FVB background, and “normal-2” lungs were from animals on a mixed genetic background (see Methods for details). (B) RT-PCR for Ereg and Areg (and actin) was performed in the presence or absence of reverse transcriptase on mRNA extracted from 3 individual normal lungs and 3 separate macrodissected tumors from C/L+T, C/T790M, and C/L858R mice, respectively. h, human; m, mouse; NI, individual normal lung. (C) Levels of mouse epiregulin and amphiregulin were measured using ELISAs (see Methods) in lysates derived from 3 individual normal lungs and 3 separate macrodissected tumors from C/L+T, C/L858R, and C/T790M mice, respectively. Data represent mean ± SEM.