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. 2009 Sep 8;119(10):2925–2941. doi: 10.1172/JCI38857

Figure 4. JNK mediates the proapoptotic role of CaMKII in ER-stressed macrophages.

Figure 4

(A and B) Macrophages were incubated under control or cholesterol-loading conditions with or without 10 μM of the JNK inhibitor SP600125 after 1 hour pretreatment, and then assayed for Fas mRNA (A) and protein (B). (C and D) Macrophages from WT or Jnk2–/– mice were incubated under control or cholesterol-loading conditions and then assayed for Fas mRNA (C) and protein (D). (E) Macrophages were incubated under control or cholesterol-loading conditions for 4 hours with or without 10 μM AIP-II after 1 hour pretreatment. One aliquot of the cell extracts was immunoblotted for phospho-JNK, total JNK, and β-actin, and another was immunoblotted for phospho-MKK4 and β-actin. (F) Macrophages were incubated with 100 ng/ml LPS for the indicated times with or without 10 μM AIP-II after 1 hour pretreatment. Phospho-JNK and total JNK were then assayed by immunoblot. (G) Macrophages from WT or Camk2g–/– mice were incubated under control or cholesterol-loading conditions and then immunoblotted for CaMKII, Fas, phospho-JNK, JNK, phospho-MKK4, MKK4, and β-actin. Differing symbols indicate P < 0.01; identical symbols indicate differences that are not significant.