Figure 7. Adoptive transfer of hT5Cyp-tranduced hCD4 cells into Rag2^–/–γc^–/– mice.

Human peripheral blood CD4⁺ T cells transduced with lentiviral vector scALPS bearing either hT5Cyp or hT5CypH126Q were sorted for GFP and injected into 6- to 10-week-old Rag2^–/–γc^–/– mice. The experimental design is shown schematically in A. (B) Flow cytometric analysis of the transduced, sorted cells prior to adoptive transfer. Left: Nearly all cells were hCD4⁺. Right: GFP fluorescence in transduced cells (open histograms, scALPS) or in untransduced, donor-identical CD4⁺ T cells (grey histogram; mock). (C) Six weeks after transplant, single-cell suspensions of thymus were analyzed for human cell engraftment and transgene expression. Left: Recipient-derived CD45⁺ mouse cells (mCD45.2) and donor-derived hCD45⁺ cells. Right: CD4 and GFP signals for the hCD45⁺ cells. In B and C, the numbers indicate the percentage of cells within the gates.