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. 2009 Oct 9;5(10):e1000617. doi: 10.1371/journal.ppat.1000617

Figure 7. Increased adherence of H. pylori to gastric epithelial cells with reduced Muc1 expression.

Figure 7

(A) Transfection of siRNAs into MKN7 epithelial cells reduced MUC1 expression on the cell surface by 80% with the 1∶1 sequence and 85% with the 1∶3 sequence compared to scrambled siRNA as determined by flow cytometry with an extracellular domain antibody BC2. (B–E) H. pylori J99wt, the CagA deletion mutant J99ΔCagA or adhesion mutant J99ΔBabAΔSabA were co-cultured at a concentration of 8×105 CFU H. pylori/well (MOI 16∶1) in a 96 well plate for 0.5 (B), 2 (C), 4.5 (D) and 20 h (E). Adhesion of H. pylori to cultured cells was determined by ELISA. Data are presented after subtraction of the mean value of the OD for gastric cells without H. pylori. Statistics: Mean±SEM (N = 6); ANOVA, post hoc: Tukey's test; scrambled siRNA vs MUC1 specific siRNA * p<0.05, ** p<0.01.