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. 2009 Oct 9;5(10):e1000617. doi: 10.1371/journal.ppat.1000617

Figure 9. Gastric epithelial cell apoptosis and necrosis is affected by MUC1/Muc1.

Figure 9

(A–D) MKN7 transfected with MUC1 siRNAs 1∶1, 1∶3 (97 and 93% knockdown compared to scrambled siRNA) and scrambled siRNA were co-cultured with 3×105 H. pylori/well (500 µL, MOI 1∶1) for 20 h (A–D). The graphs show (A) the percentage of healthy cells, (B) cells in early apoptosis (Annexin-V+, 7-AAD−), (C) late apoptosis (Annexin-V+, 7-AAD+ cells), and (D) necrosis (Annexin-V−, 7-AAD+ cells), analyzed by flow cytometry. Data were collected for three independent replicate co-cultures and are presented as mean±SD. (E) Six wild-type (Muc1+/+) and Muc1−/− mice were infected with H. pylori-SS1 as previously described [20]. The number of TUNEL positive cells per 10 random fields of view in the gastric mucosa was counted at 20× magnification. Statistics: bar = mean, Mann Whitney U test, * p<0.05. Photomicrographs of the TUNEL stained sections are shown in Figure S3.