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. 2009 Oct 9;5(10):e1000609. doi: 10.1371/journal.ppat.1000609

Figure 4. The Beclin1-binding α1 Helix of vBcl-2 is Required for Inhibition of Autophagy during γHV68 Infection.

Figure 4

(top) Representative confocal images of GFP-LC3 and HA-tagged vBcl-2 (WT and mutants) in NIH3T3 cells infected with the indicated viruses (MOI = 5). Nuclei were counterstained with 4′,6′-diamidino-2-phenylindole (DAPI). Scale bar, 5 µm. (bottom) Quantification of the percentage of virally infected cells with GFP-LC3 punctate staining. Results shown represent mean±SEM of combined results from three independent experiments (200 cells per experimental condition). *, P<0.01 versus HA-WT; **, P<0.001 versus HA-WT.