Fig. 2.
Effects of acute endotoxemia on IL-1β and TNFα mRNA expression. Macrophages and endothelial cells were isolated from livers of C3H/OuJ (white bars) or C3H/HeJ (black bars) mice 3–48 hr after administration of LPS or control (CTL). Upper panel: Samples were run on agarose gels and visualized by ethidium bromide staining. Lower panel: Samples were quantified by real time PCR. Data are presented as fold change relative to CTL. Each bar is the mean ± SD (n=8–12) from 2–3 separate experiments. *Significantly different (p<0.05) from CTL.