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. Author manuscript; available in PMC: 2009 Sep 25.
Published in final edited form as: Plant J. 2008 Sep 18;56(6):948–962. doi: 10.1111/j.1365-313X.2008.03652.x

Figure 1.

Figure 1

Glucose-induced FRET changes in the cytosol of intact roots.

(a-c) The FRET sensors FLIPglu-2μΔ13 (a), FLIPglu-600μΔ13 (b), and FLIPglu-3.2mΔ13 (c) respond to glucose perfusion in stably transformed rdr6-11 Arabidopsis roots after 5, 3 or 2 days of transfer to sugar-free medium, respectively. Quantitative data were derived by pixel-by-pixel integration of the ratiometric images. The y axis gives the ratio of eYFP intensity (ET535/30m) to eCFP intensity (ET470/24m). The bars above each graph represent the duration of perfusion with the indicated sugar concentrations.

(d-f) Saturation curves derived from the data shown in (a)-(c) for FLIPglu-2μΔ13 (d), FLIPglu-600μΔ13 (e) and FLIPglu-3.2mΔ13 (f).

(g) Confocal images showing the expression pattern of FLIPglu-600μΔ13 in the CFP, YFP and FRET channels in root tips.