Effect of protonophores on sugar response.
(a-c) Glucose accumulation in roots in the presence of 100 μM 2,4-DNP (a) or CCCP (b) measured using FLIPglu-600μΔ13, and sucrose accumulation in roots in the presence or absence of 2,4-DNP (c) measured using FLIPsuc-90μΔ1. Data were acquired as for Figure 1. A small dip in the baseline upon addition of CCCP to FLIPglu-600μΔ13 plants is shown as and inset in (a).
(d, e) Glucose response in the presence of 25 μm nigericin measured using FLIPglu-600μΔ13 (d) and sucrose response in the presence of 25 μm nigericin measured using FLIPsuc-90μΔ1 (e).