Table 1.
Microarray-identified Pbx-dependent heart genes
| Gene Symbol | Affymetrix ID number | Fold change at 10s (controlMO: pbx2-MO; pbx4-MO | Wild-type expression at 10s | RNA in situ expression in pbx2-MO; pbx4- MO at 10s | RNA in situ expression in hand2−/− or hand2- MO at 10s |
|---|---|---|---|---|---|
| tnnt2 | Dr.14260.1.S1_a_at | 1.55 | Heart primordium | Reduced | Reduced |
| ttna | Dr.4681.1.A1_at | 1.53 | Heart primordium; somites | Reduced (1) | Not affected |
| bon | Dr.8084.1.S1_at | −1.87 | Not expressed | 10X increase over control (qRT-PCR) | Similar to control (qRT-PCR) |
| sox32 | Dr.12573.1.S1_at | −1.88 | Endoderm | 3X increase over control (qRT-PCR) | Similar to control (qRT-PCR) |
| Gene Symbol | Affymetrix ID number | Fold change at 18s (controlMO: pbx2-MO; pbx4-MO | Wild-type expression at 18s | RNA in situ expression in pbx2-MO; pbx4- MO at 18s | RNA in situ expression in hand2−/− or hand2- MO at 18s |
| cmlc1 | Dr.8379.1.S1_at | 3.62 | Heart; somites | Heart domain reduced | Heart domain reduced |
| vmhc | Dr.10607.1.A1_at | 3.20 | Heart; somites | Heart domain reduced (1) | Reduced (2) |
| aldh1a2 | Dr.5206.1.S1_at | 2.26 | Lateral plate mesoderm (LPM); somites; eye | LPM domain increased; eye and somite domains reduced; increased in tail bud (1) | Not affected |
| myl7 | Dr.642.1.S1_at | 1.90 | Heart | Reduced | Reduced (2) |
| ttna | Dr.4681.1.A1_at | 1.85 | Heart; somites | Reduced (1) | Heart domain reduced |
| lft1 | Dr.8208.1.S1_at | 1.60 | Heart; forebrain; notochord | Reduced in heart and forebrain | Reduced in heart |
| hand2 | Dr.8328.1.S1_at | −1.70 | Heart; ventral mesoderm | Increased | Reduced in hand2−/− (2); Increased in hand2-MO |
| sox32 | Dr.12573.1.S1_at | −2.05 | Not expressed | 5X increase over control (qRT-PCR) | Similar to control (qRT-PCR) |