Fig. 5. SIK1 links LKB1 to p53-dependent suppression of metastasis.
(A) A549 cells expressing various versions of SIK1 with or without co-expression of shTP53 were subjected to Matrigel invasion assay. Mean ± SD for 3 independent experiments. (B) GFP-labeled A549 cells expressing various constructs as indicated were injected into tail veins of NOD/SCID mice and lung metastasis was assessed 6 weeks post injection. Representative fluorescence images of mouse lungs are shown. Lung metastasis burden was shown as indices pooled with each group of mice expressed as % (± SEM) over controls (n=6-9), * p < 0.001 (t-test). (C) Summary of LKB1-SIK1 pathway that mediates p53-dependent anoikis and suppresses metastasis.