Skip to main content
. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Bioorg Med Chem Lett. 2008 Oct 31;19(1):222–225. doi: 10.1016/j.bmcl.2008.10.107

Figure 2.

Figure 2

The catalytic mechanism of the alkaline phosphatase reaction.12 The initial alkaline phosphatase (E) catalyzed reaction consists of a substrate (DO-Pi) binding step, phosphate-moiety transfer to Ser-93 (in the TNAP sequence of its active site) and product alcohol (DOH) release. In the second step of the reaction, phosphate is released through hydrolysis of the covalent intermediate (E-Pi) and the non-covalent complex (E·Pi) of the inorganic phosphate in the active site. In the presence of nitrogen-containing alcohol molecules (AOH), such as the buffer diethanolamine (DEA), phosphate is also released via a transphosphorylation reaction.