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. Author manuscript; available in PMC: 2009 Sep 25.
Published in final edited form as: Hum Mutat. 2005 Apr;25(4):343–347. doi: 10.1002/humu.20148

Figure 3.

Figure 3

Autocatalytic activation of wild type (PRSS1), single-mutant (N29I) and double-mutant (N29I-N54S) cationic trypsinogen. 2 μM trypsinogen (final concentration, in a final volume of 100 μL) was incubated at 37 oC, in 0.1 M Tris-HCl (pH 8.0, upper panel) or 0.1 M Na-acetate buffer (pH 5.0, lower panel), in the presence of 2 mg/mL bovine serum albumin. Aliquots of 2 μL were withdrawn from reaction mixtures at indicated times and trypsin activity was determined with 0.14 mM N-CBZ-Gly-Pro-Arg-p-nitroanilide (final concentration). Activity was expressed as percentage of the potential total activity, which was determined on similar zymogen samples activated with bovine enterokinase at 22 oC in 0.1 M Tris-HCl (pH 8.0), 2 mg/mL bovine serum albumin and 1 mM CaCl2.