Skip to main content
. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Brain Behav Immun. 2009 Jun 9;23(7):1028–1037. doi: 10.1016/j.bbi.2009.06.003

Figure 1. βGBP is expressed on the surface of Tregs and secreted upon activation.

Figure 1

(A) ELISA results of βGBP in conditioned media of T cells (Tregs, non-Tregs and CD8+ T cells T cells, each 5×105) after 2 days of activation with CD3Ab. *P<0.05 versus CD8+ cells. (B) βGBP expression (red) on non-activated freshly isolated human peripheral blood Tregs or non-Tregs mixed with CFSE-labeled CD8+ T cells (green). Cells were stained with anti-Gal-1 Ab followed by Alexa 555-conjugated secondary Ab and analyzed by confocal microscopy. (C) Tregs or non Tregs were stained for βGBP as for Figure 1B after 2 days of activation with plate-bound CD3Ab and were analyzed by flow cytometry. (D) FoxP3-expressing subsets, Tregs, mostly express βGBP. Data are from intracellular staining of freshly isolated Tregs with antibodies for βGBP and FoxP3. βGBP expression is shown by thick line (C and D). Thin line represents staining with control Ab (Cnt Ab). All results were independently reproduced at least three times using different donor blood from normal humans.