Figure. Standard curves: six DNA concentrations were prepared by serial dilution (dilution factor 2) with the final amounts per reaction ranged from 2.5 to 80 ng. The Ct values in duplicate for Telo were: [17.84/17.88], [16.60/16.76], [15.52/15.48], [14.83/14.85], [13.63/13.86], and [12.35/12.64]. The Ct values in duplicate for 36B4 were: [21.84/21.80], [20.83/20.80], [19.87/19.83], [18.86/18.81], [17.78/17.77], and [16.89/17.00].