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acacTrp-S-CpaS_T and CpaS_R* activities in trans. (A) Schematized CpaS_T and CpaS_R* in trans assay procedure. (B) Time course analysis of cAATrp generation and acacTrp-SCoA consumption from acacTrp-SCoA primed CpaS_T with or without CpaS_R* present in trans.