Table 1.
Oligonucleotide primers used to generate CpaS_TR* and CpaS_R* mutants
| CpaS_TR* CpaS_R* mutant | Primer sequencea |
|---|---|
| S3508A | 5'-CTCCTCGGGGGAAATGCGACGCTGCTGGTCAGG |
| C3619A | 5'-GTGGAAAGAGTGCATGCGATCGCCGTGGAAAAGG |
| D3663A | 5'-CTGCAAGACCGGATCGCGGTGGTGATCCACAAC |
| H3667A | 5'-ATCGACGTGGTGATCGCGAACGGCTCGAATGGC |
| S3688A | 5'-TCCTCCGCTCGACGGGGCGGACGGGCTCACAGCAAC |
| S3707A | 5'-CCCCTGCACTATATCGCGTCCGGAAGGGTCATC |
| K3742A | 5'-GGGCTCACAGCAACCGCGTGGGCCAGCGAAGTC |
| D3777A | 5'-CAGGCTCCAGCACAGGCGGCACTGAACTCGCTC |
| D3803A | 5'-ATGGAAGGATACTTGGCGTTTCAGAAGGTAGAG |
| H3843A | 5'-GGTTTCCTTTTTCCACGCGTCGAGCAATGTCAAG |
a
bold: modified sequences